3EBT
Crystal structure of a ntf2-like protein of unknown function (bpss0132) from burkholderia pseudomallei k96243 at 1.30 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-01 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97848,0.97904 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.730, 75.730, 50.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.431 - 1.300 |
R-factor | 0.141 |
Rwork | 0.140 |
R-free | 0.16200 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.760 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.431 | 27.431 | 1.350 |
High resolution limit [Å] | 1.300 | 2.800 | 1.300 |
Rmerge | 0.062 | 0.049 | 0.481 |
Rmeas | 0.078 | 0.061 | 0.607 |
Total number of observations | 219462 | ||
Number of reflections | 40949 | 7739 | 8321 |
<I/σ(I)> | 9.59 | 20.6 | 2.1 |
Completeness [%] | 99.0 | 97.8 | 98.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20.0000% Glycerol, 0.0400M KH2PO4, 16.0000% PEG-8000, No Buffer pH None, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |