3EBP
Glycogen Phosphorylase b/flavopiridol complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2005-02-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.813 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.653, 128.653, 116.489 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.530 - 2.000 |
| R-factor | 0.18968 |
| Rwork | 0.188 |
| R-free | 0.21525 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1hlf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.035 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.910 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 61747 | |
| <I/σ(I)> | 17.2 | 3.8 |
| Completeness [%] | 98.8 | 99.9 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | BES, EDTA, pH 6.7, SMALL TUBES, temperature 289K |
