3EBJ
Crystal structure of an avian influenza virus protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-20 |
Detector | SBC-2 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 1 |
Unit cell lengths | 51.049, 59.825, 67.165 |
Unit cell angles | 96.55, 96.82, 109.51 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.234 |
Rwork | 0.231 |
R-free | 0.25200 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.399 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.067 | 0.490 |
Number of reflections | 31554 | |
Completeness [%] | 98.0 | 95 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 25% PEG8000 (native crystals) or 20% PEG3350, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 25% PEG8000 (native crystals) or 20% PEG3350, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |