3EB8
VirA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.290, 91.290, 198.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.990 - 2.400 |
R-factor | 0.22 |
Rwork | 0.217 |
R-free | 0.26700 |
Structure solution method | SIRAS |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.054 | 0.386 |
Number of reflections | 33386 | |
<I/σ(I)> | 27 | 2.07 |
Completeness [%] | 99.5 | 98 |
Redundancy | 6.3 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |