3EB6
Structure of the cIAP2 RING domain bound to UbcH5b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-11 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 137.226, 137.226, 111.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.520 - 3.400 |
| R-factor | 0.283 |
| Rwork | 0.281 |
| R-free | 0.32000 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.340 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.521 | 58.520 | 3.580 |
| High resolution limit [Å] | 3.401 | 10.750 | 3.400 |
| Rmerge | 0.077 | 0.010 | 0.010 |
| Total number of observations | 1912 | 8853 | |
| Number of reflections | 8512 | ||
| <I/σ(I)> | 7.331 | 17.6 | 0.8 |
| Completeness [%] | 99.1 | 93.1 | 99.6 |
| Redundancy | 6.9 | 6.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | 3.3 M NaCl, 0.1 M HEPES, pH 7.3, vapor diffusion, hanging drop, temperature 291K |
