3EB5
Structure of the cIAP2 RING domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-09-22 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.2827,1.1808,1.2829 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 34.869, 66.474, 77.465 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.690 - 2.000 |
R-factor | 0.219 |
Rwork | 0.213 |
R-free | 0.27500 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.514 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.689 | 30.540 | 2.110 |
High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
Rmerge | 0.052 | 0.034 | 0.757 |
Total number of observations | 1899 | 8036 | |
Number of reflections | 6383 | ||
<I/σ(I)> | 9.135 | 15.4 | 1 |
Completeness [%] | 100.0 | 99.3 | 99.9 |
Redundancy | 9.2 | 8 | 8.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 0.4 M NH4(SO4)2, 1 M LiSO4, 0.1 M Na Citrate, pH 6.2, vapor diffusion, hanging drop, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 0.4 M NH4(SO4)2, 1 M LiSO4, 0.1 M Na Citrate, pH 6.2, vapor diffusion, hanging drop, temperature 291K |