3EAT
Crystal structure of the PvcB (PA2255) protein from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2006-05-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 125.350, 125.350, 107.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22500 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.258 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.083 | 0.484 |
Number of reflections | 17734 | |
<I/σ(I)> | 19.1 | 2.6 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 2-5% PEG 20000, 75-150 mM NaCitrate, 100 mM BTP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |