3EAQ
Novel dimerization motif in the DEAD box RNA helicase Hera form 2, complete dimer, symmetric
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-07-31 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.620, 70.780, 101.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.880 - 2.300 |
| R-factor | 0.2207 |
| Rwork | 0.218 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | crude model built in MAD/phased maps from a tetragonal crystal form |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.069 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 20794 | |
| <I/σ(I)> | 12.9 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 293 | PEG3350, ammonium sulfate, pH 8.5, microbatch, temperature 293K |
