3EAE

PWWP domain of human hepatoma-derived growth factor 2 (HDGF2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	200
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97940
Spacegroup name	P 21 21 21
Unit cell lengths	41.555, 59.950, 104.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.240
R-factor	0.225
Rwork	0.224
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB Entries 2HTS and 2NLU
RMSD bond length	0.012
RMSD bond angle	1.261
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.340
High resolution limit [Å]	2.240	6.240	2.300
Rmerge	0.084	0.047	0.647
Number of reflections	12780
<I/σ(I)>	19.947
Completeness [%]	99.7	97.1	98.5
Redundancy	6.9	6.2	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	2.0 M NH4SO4, 0.2 M K/Na tart, 0.1 M Na Citrate pH 5.6., VAPOR DIFFUSION, HANGING DROP, temperature 298K