3E9Y
Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-10 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 178.866, 178.866, 186.078 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 89.440 - 3.000 |
| R-factor | 0.19383 |
| Rwork | 0.192 |
| R-free | 0.22348 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ybh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.106 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.440 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.072 | 0.228 |
| Number of reflections | 28796 | |
| <I/σ(I)> | 22.5 | 4.5 |
| Completeness [%] | 80.5 | |
| Redundancy | 6.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 291 | 0.1M CHES, 0.2M Li2S04, 1M potassium sodium tartrate, pH 9.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
