3E9Y
Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-12-10 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.000 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 178.866, 178.866, 186.078 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 89.440 - 3.000 |
R-factor | 0.19383 |
Rwork | 0.192 |
R-free | 0.22348 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1ybh |
RMSD bond length | 0.006 |
RMSD bond angle | 1.106 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.440 | 3.070 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.072 | 0.228 |
Number of reflections | 28796 | |
<I/σ(I)> | 22.5 | 4.5 |
Completeness [%] | 80.5 | |
Redundancy | 6.4 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 291 | 0.1M CHES, 0.2M Li2S04, 1M potassium sodium tartrate, pH 9.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |