3E9U
Crystal structure of Calx CBD2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-08 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 113.365, 113.365, 41.616 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.200 - 2.500 |
| R-factor | 0.25798 |
| Rwork | 0.257 |
| R-free | 0.27548 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.761 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.320 | 2.550 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.098 | |
| Number of reflections | 10260 | |
| <I/σ(I)> | 18.9 | 2.5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 0.2 M potassium formate, 10 mM CaCl2, 20% Polyethylene glycol 3350, pH 8.0, EVAPORATION |
