3E9T
Crystal structure of Apo-form Calx CBD1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-19 |
Detector | NOIR-1 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.086, 76.429, 128.918 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.730 - 1.600 |
R-factor | 0.19426 |
Rwork | 0.193 |
R-free | 0.22315 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.634 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.600 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 73482 | |
<I/σ(I)> | 15.6 | 2 |
Completeness [%] | 98.7 | 95.8 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 291 | 100 mM Bis-Tris, 200 mM NH4Ac, 10 mM MgCl2, 15% Polyethylene glycol 10,000, pH 6.5, EVAPORATION, temperature 291K |