3E9P
Crystal Structure of Yeast Prp8, Residues 1827-2092
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97888 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.117, 84.161, 95.054 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.23991 |
| Rwork | 0.237 |
| R-free | 0.29799 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.216 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 34578 | |
| Completeness [%] | 99.6 | 99.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
