3E9O
Crystal Structure of Yeast Prp8, Residues 1836-2092
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97968, 0.97987, 0.91841 |
| Spacegroup name | P 31 |
| Unit cell lengths | 46.857, 46.857, 102.995 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.18011 |
| Rwork | 0.177 |
| R-free | 0.24515 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.146 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 17446 | |
| Completeness [%] | 96.8 | 88.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
