3E9D
Structure of full-length TIGAR from Danio rerio
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-14 |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 127.661, 115.541, 61.311 |
Unit cell angles | 90.00, 104.66, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.173 |
Rwork | 0.171 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.140 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | COMO |
Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.076 | 0.033 | 0.425 |
Number of reflections | 57061 | ||
Completeness [%] | 96.1 | 98.1 | 79.3 |
Redundancy | 3 | 3.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 1 M sodium phosphate, pH 6.5, vapor diffusion, temperature 293K |