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3E8G

Crystal Structure of the the open NaK channel-Na+/Ca2+ complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-ID
Synchrotron siteAPS
Beamline19-ID
Temperature [K]100
Detector technologyCCD
DetectorADSC QUANTUM 315
Spacegroup nameI 4
Unit cell lengths67.990, 67.990, 89.168
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution33.990 - 2.000
Rwork0.202
R-free0.23500
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.006
RMSD bond angle1.099
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareMOLREP
Refinement softwareCNS
Data quality characteristics
 Overall
Low resolution limit [Å]50.000
High resolution limit [Å]2.000
Number of reflections13398
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.5293100mM Hepes, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 10mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

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PDB entries from 2024-10-30

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