3E8G
Crystal Structure of the the open NaK channel-Na+/Ca2+ complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 4 |
Unit cell lengths | 67.990, 67.990, 89.168 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.990 - 2.000 |
Rwork | 0.202 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.099 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 13398 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100mM Hepes, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 10mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |