3E8F

Crystal Structure of the the open NaK channel- K+/Ba2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Detector	SBC-3
Spacegroup name	I 4
Unit cell lengths	68.226, 68.226, 89.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.880 - 2.000
Rwork	0.201
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.068
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	13987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	100mM Hepes Buffer, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), Soaked in stabilization solution containing 67mM KCl and 33mM BaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K