3E7L
Crystal structure of sigma54 activator NtrC4's DNA binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.28237 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.239, 55.866, 62.338 |
| Unit cell angles | 90.00, 93.39, 90.00 |
Refinement procedure
| Resolution | 41.167 - 2.252 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24300 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.701 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.170 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.062 | 0.062 |
| Number of reflections | 13286 | |
| <I/σ(I)> | 16.5 | 3.9 |
| Completeness [%] | 98.2 | 94.7 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10mM CdSO4, 20% PEG 550 MME, and 100mM MES pH 6.5, vapor diffusion, hanging drop, temperature 298K |
