3E7K
Crystal Structure of an antiparallel coiled-coil tetramerization domain from TRPM7 channels
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-19 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9796 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.258, 35.674, 100.322 |
Unit cell angles | 90.00, 124.46, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.010 |
R-factor | 0.191 |
Rwork | 0.188 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.783 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.121 | 0.359 |
Number of reflections | 28234 | |
Completeness [%] | 97.7 | 85.4 |
Redundancy | 3.3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 0.1 M MgCl2, 0.1 M Tris, 5-10% Isopropanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |