3E7H
The crystal structure of the beta subunit of the DNA-directed RNA polymerase from Vibrio cholerae O1 biovar eltor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2008-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.035, 65.035, 115.981 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.980 - 1.700 |
| R-factor | 0.18301 |
| Rwork | 0.181 |
| R-free | 0.22906 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.471 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.980 | 1.744 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.081 | 0.727 |
| Number of reflections | 26715 | |
| <I/σ(I)> | 50.5 | 2.58 |
| Completeness [%] | 1.0 | 0.995 |
| Redundancy | 18.5 | 17.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 25% PEG3350, 0.1M Bis-tris, pH6.5, 0.2M NaCl , VAPOR DIFFUSION, SITTING DROP, temperature 298K |
