3E78
Structure determination of the cancer-associated Mycoplasma hyorhinis protein Mh-p37
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2008-05-05 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.445, 68.761, 60.024 |
Unit cell angles | 90.00, 108.04, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.900 |
R-factor | 0.186 |
R-free | 0.24000 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 0.022 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.980 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 29894 | |
<I/σ(I)> | 16.3 | 5 |
Completeness [%] | 96.2 | 93.2 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch | 3 | 298 | 0.1 M Ammonium Bromide, O.1 M Citric Acid, 40% PEG 4000, 9% N-decyl-beta-D-maltoside detergent , pH 3.0, Batch, temperature 298K |