3E77
Human phosphoserine aminotransferase in complex with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 171.000, 171.000, 103.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.590 - 2.500 |
| R-factor | 0.19875 |
| Rwork | 0.196 |
| R-free | 0.24409 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.371 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.093 | 0.656 |
| Number of reflections | 60117 | |
| <I/σ(I)> | 10.47 | 2.08 |
| Completeness [%] | 99.0 | 98.9 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.1 M Malic acid, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
