3E6Z
1.0 A Structure of CusF-W44A-Cu(II) residues 10-88 from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-02 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.272, 31.615, 84.685 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.330 - 1.000 |
| R-factor | 0.19536 |
| Rwork | 0.194 |
| R-free | 0.21393 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qcp |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.315 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.330 | 1.030 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.044 | 0.035 |
| Number of reflections | 35801 | |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 96.0 | 90.9 |
| Redundancy | 6.9535801 | 6.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M Tris-HCl pH 8.5, 27.5% PEG 4000, 0.2 M sodium acetate, 2 mM CuCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
