3E6S
Crystal structure of ferritin soaked with iron from Pseudo-nitzschia multiseries
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-05-31 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 125.625, 125.625, 170.132 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.220 - 1.950 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20500 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.414 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.064 | 0.463 |
| Number of reflections | 99298 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Crystals grown in 0.1 M ammonium acetate pH 5.5 and 1.6 M ammonium sulphate then soaked in 20 mM ferrous sulphate, 2 mM sodium sulphate and 25% glycerol for 10 minutes, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
