3E6M
The crystal structure of a MarR family transcriptional regulator from Silicibacter pomeroyi DSS.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.433, 93.198, 101.556 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 39.600 - 2.200 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.23030 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.223 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.600 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.695 |
| Number of reflections | 136295 | |
| <I/σ(I)> | 20.9 | 1.54 |
| Completeness [%] | 97.8 | 84.3 |
| Redundancy | 4.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 289 | 20% PEG1000, 0.1M Phosphate-citrate 0.2M Li2SO4, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
