3E5Y

Crystal structure of TrmH family RNA methyltransferase from Burkholderia pseudomallei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-02
Detector	RIGAKU SATURN 944
Spacegroup name	P 21 21 21
Unit cell lengths	46.724, 80.532, 80.882
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.215
Rwork	0.213
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j85
RMSD bond length	0.017
RMSD bond angle	1.724
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.4.0067)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.140	0.652
Number of reflections	12194
<I/σ(I)>	12	3
Completeness [%]	97.9	95.9
Redundancy	6.4	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	289	PACT sparse matrix screen G10 and H10; 20% PEG 3350, 0.2 M sodium/potassium phosphate, 0.1 M BisTris propane, pH 7.5 or 8.5; 27.6 mg/mL protein; Crystal ID 109613h10, VAPOR DIFFUSION, SITTING DROP, temperature 289K