3E5R
Crystal structure and Functional Analysis of Glyceraldehyde-3-phosphate Dehydrogenase from Oryza Sativa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-09 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.445, 129.558, 77.578 |
| Unit cell angles | 90.00, 117.40, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| Rwork | 0.199 |
| R-free | 0.23900 |
| Starting model (for MR) | 1u8f |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 57264 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 293 | 0.1M citric acid, 16%(w/v) PEG3350, pH4.1, vapor diffusion, hanging drop, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
