3E5M
Crystal structure of the HSCARG Y81A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-11 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.956, 89.036, 84.182 |
| Unit cell angles | 90.00, 88.23, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.26081 |
| Rwork | 0.260 |
| R-free | 0.27267 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2exx |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.043 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 60.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 22513 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 3% MPD, 10mM potassium chloride, 0.1M sodium Hepes, 1.86M ammonium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
