3E5D

Crystal structure of a putative glyoxalase i (lmof2365_0426) from listeria monocytogenes str. 4b f2365 at 2.70 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-27
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	I 21 3
Unit cell lengths	123.590, 123.590, 123.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.136 - 2.700
R-factor	0.173
Rwork	0.172
R-free	0.19800
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.657
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.136	29.136	2.800
High resolution limit [Å]	2.700	5.770	2.700
Rmerge	0.051	0.034	0.991
Number of reflections	8775	1722	1700
<I/σ(I)>	22.2	44.1	2.2
Completeness [%]	99.7	98.7	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	1.1M sodium citrate, 0.15M sodium chloride, 0.1M TRIS pH 7.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP