3E59
Crystal structure of the PvcA (PA2254) protein from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2008-02-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.226, 132.530, 96.897 |
Unit cell angles | 90.00, 97.93, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.19955 |
Rwork | 0.197 |
R-free | 0.24618 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.191 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.053 | 0.314 |
Number of reflections | 77408 | |
<I/σ(I)> | 16.5 | |
Completeness [%] | 93.6 | 0.618 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9 | 293 | 41-45% PEG 1000, 35-50 mM KH2PO4, 100 mM TrisHCl, pH 9.0, VAPOR DIFFUSION, temperature 293K |
1 | VAPOR DIFFUSION | 9 | 293 | 41-45% PEG 1000, 35-50 mM KH2PO4, 100 mM TrisHCl, pH 9.0, VAPOR DIFFUSION, temperature 293K |
1 | VAPOR DIFFUSION | 9 | 293 | 41-45% PEG 1000, 35-50 mM KH2PO4, 100 mM TrisHCl, pH 9.0, VAPOR DIFFUSION, temperature 293K |