3E43
Q138F HincII bound to GTTAAC and cocrystallized with 2.5 mM MgCl2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Collection date | 2007-07-13 |
Wavelength(s) | 0.913321 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.271, 90.938, 66.526 |
Unit cell angles | 90.00, 105.14, 90.00 |
Refinement procedure
Resolution | 44.741 - 2.730 |
R-factor | 0.196 |
Rwork | 0.192 |
R-free | 0.27500 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.944 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.730 | 2.730 |
Rmerge | 0.085 | 0.490 |
Number of reflections | 14388 | |
<I/σ(I)> | 9.7 | 2 |
Completeness [%] | 93.4 | 94.7 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | PEG 4000, 150 mM NaCl, 10 mM HEPES pH 7.5, 2.5 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | HEPES | ||
4 | 1 | 1 | MgCl2 | ||
5 | 1 | 2 | PEG 4000 | ||
6 | 1 | 2 | NaCl | ||
7 | 1 | 2 | HEPES | ||
8 | 1 | 2 | MgCl2 |