3E3Z
Crystal structure of bovine coupling Factor B bound with phenylarsine oxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.122, 49.722, 36.902 |
| Unit cell angles | 90.00, 108.84, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dze |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.410 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.039 | 0.027 | 0.113 |
| Number of reflections | 19212 | ||
| Completeness [%] | 95.6 | 98.3 | 78.3 |
| Redundancy | 3.6 | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 277 | 50% PPG 400, 100 mM Tris-HCl pH 8.0, VAPOR DIFFUSION, temperature 277K |
