3E3X
The C-terminal part of BipA protein from Vibrio parahaemolyticus RIMD 2210633
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2008-07-30 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 90.600, 104.571, 74.348 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.950 |
R-factor | 0.174 |
Rwork | 0.172 |
R-free | 0.22300 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.845 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.090 | 0.560 |
Number of reflections | 25871 | |
<I/σ(I)> | 29.8 | 4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 277 | 0.1 M Hepes, 20 % PEG 4k 10% Isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |