3E3V
Crystal structure of RecX from Lactobacillus salivarius
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 164.910, 42.712, 24.185 |
Unit cell angles | 90.00, 91.50, 90.00 |
Refinement procedure
Resolution | 33.720 - 2.040 |
R-factor | 0.247 |
Rwork | 0.247 |
R-free | 0.25800 |
Structure solution method | SAD |
Starting model (for MR) | None |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 2.040 | 2.040 |
Rmerge | 0.060 | 0.500 |
Number of reflections | 10853 | |
<I/σ(I)> | 11.3 | 2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 9.9 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 2M Ammonium sulfate, 0.1M Na-citrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |