3E3O
Glycogen phosphorylase R state-IMP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8063 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.680, 188.448, 87.853 |
| Unit cell angles | 90.00, 109.10, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.20405 |
| Rwork | 0.201 |
| R-free | 0.26703 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2abb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.359 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 105631 | |
| <I/σ(I)> | 7.07 | 2.62 |
| Completeness [%] | 99.5 | 96.4 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.5 | 289 | 1.1-1.3M ammonium sulfate, 10mM beta-glycerophosphate buffer pH 7.5, 0.5mM EDTA, MICRODIALYSIS, temperature 289K |
