3E3L
The R-state Glycogen Phosphorylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2008-02-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.895, 189.920, 88.160 |
| Unit cell angles | 90.00, 109.27, 90.00 |
Refinement procedure
| Resolution | 29.590 - 2.590 |
| R-factor | 0.20958 |
| Rwork | 0.207 |
| R-free | 0.26577 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 9gpb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.341 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.590 | 2.600 |
| Number of reflections | 107281 | |
| <I/σ(I)> | 11.7 | 3.89 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 7.5 | 289 | 1.1-1.3 M ammonium sulfate, 10mM beta-glycerophosphate buffer pH 7.5, 0.5mM EDTA, SMALL TUBES, temperature 289K |
