3E2B
Crystal structure of Dynein Light chain LC8 in complex with a peptide derived from Swallow
Replaces: 2P1KExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 77 |
| Detector technology | AREA DETECTOR |
| Collection date | 2008-02-27 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 44.158, 44.158, 203.718 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.810 - 2.000 |
| R-factor | 0.22084 |
| Rwork | 0.216 |
| R-free | 0.26599 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p1k |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.880 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.700 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 10184 | |
| <I/σ(I)> | 23.5 | 5.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.7 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2M sodium potassium tartrate, 0.1M sodium citrate, 2.0M ammonium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
