3E23

Crystal structure of the RPA2492 protein in complex with SAM from Rhodopseudomonas palustris, Northeast Structural Genomics Consortium Target RpR299

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97902
Spacegroup name	P 21 21 21
Unit cell lengths	53.185, 65.295, 67.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.860 - 1.600
R-factor	0.16
Rwork	0.160
R-free	0.18300
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELX (then SOLVE/RESOLVE)
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.049	0.074
Number of reflections	54164
<I/σ(I)>	33.94	12.77
Completeness [%]	90.2	66.3
Redundancy	4.1	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	277	Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, 5 mM DTT, and 5 mM S-adenosyl-methionine. Resevoir solution: 100 mM HEPES (pH 7.5) and 2 M ammonium sulfate. , microbatch under oil, temperature 277K