3E20

Crystal structure of S.pombe eRF1/eRF3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-30
Detector	MAR CCD 165 mm
Wavelength(s)	0.9790
Spacegroup name	P 43
Unit cell lengths	129.848, 129.848, 332.638
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.500
R-factor	0.25915
Rwork	0.258
R-free	0.27987
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dt9 1r5b
RMSD bond length	0.007
RMSD bond angle	1.110
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.4.0077)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.620
High resolution limit [Å]	3.500	3.500
Rmerge	0.078	0.560
Number of reflections	69011
<I/σ(I)>	8
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	288	50mM MOPS, pH7.0, 500mM KCl, 12% PEG4000, 20% Glycerol, EVAPORATION, temperature 288K