3E1U
The Crystal Structure of the Anti-Viral APOBEC3G Catalytic Domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792060, 0.9790670 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.464, 57.329, 40.578 |
| Unit cell angles | 90.00, 96.46, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.252 |
| Rwork | 0.251 |
| R-free | 0.26700 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 17604 | |
| Completeness [%] | 93.1 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100mM MES pH 6.5, 40% PEG 200, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
