3E1T
Structure and action of the myxobacterial chondrochloren halogenase CndH, a new variant of FAD-dependent halogenases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.918, 99.213, 101.774 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.900 - 2.050 |
| R-factor | 0.22316 |
| Rwork | 0.222 |
| R-free | 0.27075 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.204 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.900 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 33613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 100mM HEPES, 26% (v/v) PEG 400, 200mM MgCl2, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
