3E1S
Structure of an N-terminal truncation of Deinococcus radiodurans RecD2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.07225 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.630, 89.630, 131.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.700 - 2.200 |
| R-factor | 0.23541 |
| Rwork | 0.231 |
| R-free | 0.28744 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.246 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.038 | 0.321 |
| Number of reflections | 32947 | |
| <I/σ(I)> | 15.2 | 3.2 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 2.4 | 2.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 13-18% ethanol, 100mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
