3E1O
Crystal structure of E. coli Bacterioferritin (BFR) with two ZN(II) ION sites at the Ferroxidase centre (ZN-BFR).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-01-01 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 207.867, 207.867, 142.432 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.810 - 2.950 |
R-factor | 0.23 |
Rwork | 0.229 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2QP7 |
RMSD bond length | 0.022 |
RMSD bond angle | 1.941 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 3.110 |
High resolution limit [Å] | 2.950 | 2.950 |
Number of reflections | 58778 | |
<I/σ(I)> | 20.9 | 7.9 |
Completeness [%] | 89.2 | 89.2 |
Redundancy | 9.1 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | APO-BFR CRYSTALIZED IN 1.8 M AMMONIUM SULFATE, 0.1 M TRI-SODIUM CITRATE PH 5.0. CRYSTAL WAS THEN SOAKED IN A CRYOPROTECTANT CONTAINING ZN2+ IONS AND BUFFERED WITH MOPS PH 7 INSTEAD OF CITRATE. PLEASE SEE PAPER FO FULL DETAILS., VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 298K, pH 5.00 |