3E1K
Crystal structure of Kluyveromyces lactis Gal80p in complex with the acidic activation domain of Gal4p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-06 |
Detector | SBC-3 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 101.100, 160.500, 132.600 |
Unit cell angles | 90.00, 94.70, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.000 |
R-factor | 0.231 |
Rwork | 0.228 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nvw |
RMSD bond length | 0.009 |
RMSD bond angle | 2.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.098 | 0.249 |
Number of reflections | 75005 | |
<I/σ(I)> | 7.2 | 2 |
Completeness [%] | 88.5 | 55.8 |
Redundancy | 2.4 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM MES, 20-25% (w/v) pentaerythritol propoxylate 5/4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |