3E17
Crystal structure of the second PDZ domain from human Zona Occludens-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-10-02 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 30.177, 41.325, 41.292 |
Unit cell angles | 80.05, 68.63, 68.52 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fne |
RMSD bond length | 0.014 |
RMSD bond angle | 1.498 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.750 |
Number of reflections | 15928 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 283 | 12% PEG8000, 0.1M Tris-HCl, pH8.3, VAPOR DIFFUSION, HANGING DROP, temperature 283K |