3E15
6-phosphogluconolactonase from Plasmodium vivax
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.524, 80.846, 89.765 |
| Unit cell angles | 63.98, 88.10, 80.01 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.20200 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.092 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | SOLVE (2.12) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 80.582 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.045 |
| Number of reflections | 76585 |
| Completeness [%] | 98.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 298 | 17% PEG 1000, 0.1 M potassium chloride, 0.1 M sodium acetate, 5 mM DTT, pH 5.0, vapor diffusion, temperature 298K |
