3E13
Iron reconstituted ferric binding protein from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-3 |
| Synchrotron site | SSRL |
| Beamline | BL11-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.974609 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.113, 54.740, 92.237 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.800 - 1.600 |
| R-factor | 0.14677 |
| Rwork | 0.145 |
| R-free | 0.18595 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y4t |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.581 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.106 | 0.196 |
| Number of reflections | 38962 | |
| <I/σ(I)> | 8.6 | 3 |
| Completeness [%] | 90.9 | 73.9 |
| Redundancy | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 24% PEG 4000, 0.1M Tris-HCl, 60 mM sodium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
