3E0Z

Crystal structure of a putative imidazole glycerol phosphate synthase homolog (eubrec_1070) from eubacterium rectale at 1.75 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-15
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97895
Spacegroup name	P 1 21 1
Unit cell lengths	51.790, 60.180, 59.860
Unit cell angles	90.00, 98.18, 90.00

Refinement procedure

Resolution	29.656 - 1.750
R-factor	0.174
Rwork	0.171
R-free	0.21900
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.817
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.656	29.660	1.800
High resolution limit [Å]	1.750	7.830	1.750
Rmerge	0.136	0.055	0.537
Total number of observations		1896	6666
Number of reflections	36433
<I/σ(I)>	4.5	7.5	1.4
Completeness [%]	99.0	98.1	97.7
Redundancy	4.4	4.3	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	30.0000% PEG-6000, 0.1M MES pH 6.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP
1	VAPOR DIFFUSION, SITTING DROP	6	277	30.0000% PEG-6000, 0.1M MES pH 6.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP