3E0Y
The crystal structure of a conserved domain from a protein of Geobacter sulfurreducens PCA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97945, 0.97959 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 59.421, 59.421, 223.175 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.300 - 3.100 |
| R-factor | 0.21983 |
| Rwork | 0.216 |
| R-free | 0.29089 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.901 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.300 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.089 | |
| Number of reflections | 8896 | |
| <I/σ(I)> | 23.7 | 1.7 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 6.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 15%(v/v) ethanol, 0.1M HEPES, 0.2M MgCl., pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
