3E0U
Crystal structure of T. cruzi GPX1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.846, 46.591, 61.163 |
| Unit cell angles | 90.00, 121.54, 90.00 |
Refinement procedure
| Resolution | 25.420 - 2.300 |
| R-factor | 0.20951 |
| Rwork | 0.207 |
| R-free | 0.25368 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology model based on 2P5R all loops deleted |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.933 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.470 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 7515 | |
| Completeness [%] | 94.1 | 85.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.9 M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
